Both a thorough introduction and laboratory guide! This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing. The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.

Roland Kellner is the author ofMicrocharacterization of Proteins, published by Wiley.

Friedrich Lottspeich is the author ofMicrocharacterization of Proteins, published by Wiley.

Contents Section I: Overview Microcharacterization of Proteins Friedrich Lottspeich Section II: Sample Preparation Microseparation Techniques I: 2D-Gel-Electrophoresis A. Görg and Reiner Westermeier Microseparation Techniques II: Electroblotting Christoph Eckerskorn Microseparation Techniques III: Analysis of Peptides and Proteins by Capillary Electrophoresis Christine Schwer Microseparation Techniques IV: High Performance Liquid Chromotography Maria Serwe and Helmut E. Meyer Chemical and Enzymatic Fragmentation of Proteins Roland Kellner Section III: Amino Acid Analysis Amino Acid Analysis Roland Kellner, Helmut E. Meyer and Friedrich Lottspeich Section IV: Protein Sequence Analysis The Edman Degradation Friedrich Lottspeich, Tony Houthaeve and Roland Kellner Analyzing Post translational Protein Modifications Helmut E. Meyer Section V: Bioanalytical Mass Spectrometry Overview, Methods and Applications W.D. Lehmann Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Ute Bahr, Michael Karas and Franz Hillenkamp Electrospray Mass Spectrometry Jörg Metzger and Christoph Eckerskorn Sequence Analysis of Proteins and Peptides by Mass Spectrometry Christiane Weigt, Helmut E. Meyer and Roland Kellner Section VI: Database Analysis Protein Sequences and Sequence Databases Hans-Werner Mewes and David G. George Mass Spectrometrical Data for Protein Sequence Analysis Matthias Mann Computational Sequence Analysis R. Apple and Denis F. Hochstrasser Index