The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.

InhaltsangabeSECTION I: EXPLORING ANIMAL PROTEOMES.

1. General Introduction.

The basis of proteomics to provide additional perspectives in animal biology. This chapter will also set proteomics within the context of animal genome programmes and provide an outline of different proteomic strategies..

2. Types of Samples and Experimental Planning.

The types of samples used in animal proteomics experiments and the methodologies routinely used in the preparation of samples from the main body fluids and tissues of interest (blood, blood cells, muscle, liver, kidney, lung, intestine. CNS, CSF, urine, milk, venom). The analysis of proteins from different cellular fractions will be discussed..

3. Protein Separation Strategies.

This will cover electrophoretic and chromatographic techniques used for protein separation and isolation such as 1D and 2D SDS-PAGE, liquid chromatography, ion exchange chromatography and size exclusion chromatography..

4. Methods for Protein Identification.

This chapter will discuss the mass spectrometric analyses in protein identification. This will cover the analyses intact proteins, the use of peptide mass fingerprinting and de novo sequencing strategies. The variety of instrument platforms routinely used in proteomics experiments will be also described, for example MALDI-TOF-MS, LC-MS/MS and MudPIT. The use of antibody-based approaches such as ELISA, protein arrays and western blotting to confirm protein identities will be explored..

5. Comparative Proteomic Approaches.

This chapter will discuss the theoretical and practical aspects of methods used in comparative proteomic analysis. This will cover gel-based strategies including the densitometric analysis of 1D and 2D gels and DIGE and the use of mass spectrometric approaches to determine the relative and absolute concentrations of proteins. The evaluation and interpretation of the quantitative proteomic data and the use of univariate and multivariate statistical analysis will also be discussed..

6. Advancing Technologies for Spatial and Temporal Proteomics.

This chapter will outline a number of emerging technologies that have been developed for proteome simplification, protein turnover, post-translational modifications and protein-protein interactions. The ability of these approaches to explore animal proteomes greater depth will be described and how they can provide functional