As in the second edition, the third edition ofHandbook of Practical Immunohistochemistry Frequently Asked Questions is written in a question and answer (Q&A) format and intended to be a practical, user-friendly, quick reference for information related to using the most up-to-date immunohistochemistry and in situ hybridization in clinical diagnosis. The new edition demonstrates a significant revision and improvement over the second edition. Five new chapters have been added that cover the following: 1) Immunohistochemistry: Leicas perspective; 2) Immunohistochemistry: Maixin perspective; 3) RNA in situ hybridization: Applications in anatomic pathology; 4) Applications of rapid immunohistochemistry on frozen tissue sections during intraoperative pathologic diagnosis; and 5) Cutaneous lymphomas. In addition to extensive additions and changes, over 150 new questions and answers were added throughout this new edition. All chapters have been updated to include relevant new questions, new markers, more refined IHC panels, representative pictures, and current references.  An extensive set of high-quality color pictures and diagnostic algorithms, if available, is included in each chapter to illustrate some of the key antibodies, including many recently discovered and substantiated antibodies used in that chapter.Written by experts in the field,Handbook of Practical Immunohistochemistry Third Edition is a comprehensive and practical guide for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

Fan LinDepartment of Laboratory MedicineGeisinger Medical CenterDanville, PAUSA

Jeffrey W. PrichardDepartment of Laboratory MedicineGeisinger Medical CenterDanville, PAUSA

Haiyan LiuDepartment of Laboratory MedicineGeisinger Medical CenterDanville, PAUSA

Myra L. WilkersonDepartment of Laboratory MedicineGeisinger Medical CenterDanville, PAUSA

1. Immunohistochemistry Quality Management And Regulation.- 2. Standardization of Diagnostic Immunohistochemistry.- 3. Automated Immunohistochemistry Overview.- 4. Immunohistochemistry: An Agilent Perspective.- 5. The Leica Biosystems Perspective : From excision to imaging every step is critical.- 6. Immunohistochemistry: Maixin Perspective.- 7. Immunohistochemistry: Roche Tissue Diagnostics Perspective.- 8. Applications Of Rapid Immunohistochemistry On Frozen Tissue Sections During Intraoperative Pathological Diagnoses.- 9. RNA In Situ Hybridization:  Applications in Anatomic Pathology.- 10. Overview of Immunohistochemistry Assessment of Cancer-Related Predictive Biomarkers and Common Genetic Alterations.- 11. Tissue Microarray.- 12. Unknown Primary/Undifferentiated Neoplasm.- 13. Exfoliative Cytology And Effusions.- 14. Breast.- 15. Predictive Biomarkers In Breast Cancer:  ER, PR And HER-2/NEU.- 16. Central Nerve System.- 17. Thyroid, Parathyroid, and Adrenal glands.- 18.Salivary Glands and Head and Neck.- 19. Pleuropulmonary And Mediastinal Neoplasms.- 20. Uterus.- 21. Ovary.- 22. Prostate Gland.- 23. Urinary Bladder And Urachus.- 24. Kidney.- 25. Testis And Paratesticular Tissues.- 26. Pancreas And Ampulla.- 27. Liver, Bile Ducts And GallBladder.- 28. Upper Gastrointestinal Tract.- 29. Lower Gastrointestinal Tract And Microsatellite Instability (MSI).- 30. Soft Tissue and Bone Tumors.- 31. Lymph Node.- 32. Bone Marrow.- 33. Cutaneous Lymphomas.- 34. Infectious diseases.- 35. Skin.- 36. Application of Direct Immunofluorescence for Skin and Mucosal Biopsies:  A Practical Review.- 37. Application Of Fluorescent In Situ Hybridization (FISH) In Surgical And Cytologic Specimens (Solid Tumors, Hematopoietic Tumors, Urine, Bile Duct Brushing And Bronchoscopy).