Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

Professor Schmidt-Traub was Professor for Plant and Process Design at the Department of Biochemical and Chemical Engineering, University of Dortmund, Germany until his retirement in 2006. He is still active in the research community and his main areas of research focus on preparative chromatography, down stream processing, integrated processes, plant design and innovative energy transfer. Prior to his academic appointment, Prof. Schmidt-Traub gained 15 years of industrial experience in plant engineering. Prof. Seidel-Morgenstern is the Director of the Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany and holds the Chair in Chemical Process Engineering at the Otto-von-Guericke-Universitat, Magdeburg, Germany. He received his Ph.D. in 1987 at the Institute of Physical Chemistry of the Academy of Sciences in Berlin. From there he went on to work as postdoctoral fellow at the University of Tennessee, Knoxville, USA. In 1994 he finished his habilitation at the Technical University in Berlin. His research is focused on New Reactor Concepts, Chromatographic Reactors, Membrane Reactors, Adsorption and Preparative Chromatography and Separation of Enantiomers amongst others. Dr. Michael Schulte is Senior Director Emerging Businesses Energy at Merck KGaA Performance Materials, Darmstadt, Germany. In his Ph.D. thesis at the University of Munster, Germany he developed new chiral stationary phases for chromatographic enantioseparations. In 1995 he joined Merck and has since then been responsible for research and development in the area of preparative chromatography, including the development of new stationary phases, new separation processes and the implementation of Simulated Moving Bed-technology at Merck. In his current position one of his areas of research is the use of Ionic Liquids for separation processes.

Preface INTRODUCTION On the Development of Chromatography Focus of the Book Recommendation to read this Book FUNDAMENTALS AND GENERAL TERMINOLOGY Principles of Adsorption Chromatography Basic Effects and Chromatographic Definitions Fluid Dynamics Mass Transfer Phenomena Equilibrium Thermodynamics Thermodynamic Effects on Mass Separation STATIONARY PHASES AND CHROMATOGRAPHIC SYSTEMS Column Packings Selection of Chromatographic Systems CHROMATOGRAPHY EQUIPMENT: ENGINEERING AND OPERATION Introduction Engineering and Operational Challenges Chromatography Columns Market Chromatography Systems Market Process Control Packing Methods Process Troubleshooting Disposable Technology for Bio-Separations PROCESS CONCEPTS Discontinuous Processes Continuous Processes Choice of Process Concepts MODELING AND MODEL PARAMETERS Introduction Models for Single Chromatographic Columns Modeling HPLC plants Calculations Methods Parameter Determination Experimental Validation of Column Models MODEL BASED DESIGN, OPTIMIZATION AND CONTROL Basic Principles and Definitions Batch Chromatography Recycling Chromatography Conventional Isocratic SMB Chromatography Isocratic SMB Chromatography under Variable Operating Parameters Gradient SMB Chromatography Multicolumn Systems for Bioseparations Chromatographic SMB Reactors Advanced Process Control APPENDIX B Data of Test Systems