The eight-volume set LNCS 13431, 13432, 13433, 13434, 13435, 13436, 13437, and 13438 constitutes the refereed proceedings of the 25th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2022, which was held in Singapore in September 2022. The 574 revised full papers presented were carefully reviewed and selected from 1831 submissions in a double-blind review process. The papers are organized in the following topical sections:Part I: Brain development and atlases; DWI and tractography; functional brain networks; neuroimaging; heart and lung imaging; dermatology;Part II: Computational (integrative) pathology; computational anatomy and physiology; ophthalmology; fetal imaging;Part III: Breast imaging; colonoscopy; computer aided diagnosis;Part IV: Microscopic image analysis; positron emission tomography; ultrasound imaging; video data analysis; image segmentation I;Part V: Image segmentation II; integration of imaging with non-imaging biomarkers;Part VI: Image registration; image reconstruction;Part VII: Image-Guided interventions and surgery; outcome and disease prediction; surgical data science; surgical planning and simulation; machine learning - domain adaptation and generalization;Part VIII: Machine learning - weakly-supervised learning; machine learning - model interpretation; machine learning - uncertainty; machine learning theory and methodologies.