Summarizes the essential biosynthetic pathways for assembly of metal cofactor sites in functional metalloproteins
Metalloprotein Active Site Assembly focuses on the processes that have evolved to orchestrate the assembly of metal cofactor sites in functional metalloproteins. It goes beyond the simple incorporation of single metal ions in a protein framework, and includes metal cluster assembly, metal-cofactor biosynthesis and insertion, and metal-based post-translational modifications of the protein environments that are necessary for function. Several examples of each of these areas have now been identified and studied; the current volume provides the current state-of-the-art understanding of the processes involved.
An excellent companion to the earlier book in this series Metals in Cellswhich discussed both the positive and negative effects of cellular interactions with metalsthis comprehensive book provides a diverse sampling of what is known about metalloprotein active site assembly processes. It covers all major biological transition metal components (Mn, Fe, Co, Ni, Mo), as well as the other inorganic components, metal-binding organic cofactors (e.g., heme, siroheme, cobalamin, molybdopterin), and post-translationally modified metal binding sites that make up the patchwork of evolved biological catalytic sites. The book compares and contrasts the biosynthetic assembly of active sites involving all biological metals. This has never been done before since it is a relatively new, fast-developing area of research.
Metalloprotein Active Site Assembly is an ideal text for practitioners of inorganic biochemistry who are studying the biosynthetic pathways and gene clusters involved in active site assembly, and for inorganic chemists who want to apply the concepts learned to potential synthetic pathways to active site mimics.
Contributors ix
Series Preface xiii
Volume Preface xv
Part 1: Assembly and Trafficking of Simple Fe-S Clusters 1
Nif System for Simple [FeS] Cluster Assembly in Nitrogen-Fixing Bacteria 3Patricia C. Dos Santos and Dennis R. Dean
IronSulfur Cluster Assembly in Bacteria and Eukarya using the ISC Biosynthesis Machinery 17Sandrine Ollagnier de Choudens and Hélène Puccio
The Suf System in Archaea, Bacteria, and Eukaryotic Organelles 37Guangchao Dong, Savannah Witcher, F. Wayne Outten and Marinus Pilon
Roles of Class II Glutaredoxins in the Maturation of FeS Proteins 53Jonathan Przybyla-Toscano, Thomas Roret, Jérémy Couturier and Nicolas Rouhier
Part 2: Assembly of Complex and Heterometallic Fe-S Cluster Active Sites 73
Nitrogenase Metallocluster Assembly 75Nathaniel S. Sickerman, Lee A. Rettberg, Yilin Hu and Markus W. Ribbe
Metallocluster Assembly: Maturation of [FeFe]-Hydrogenases 93Giorgio Caserta, Ludovic Pecqueur, Cecilia Papini and Marc Fontecave
CO Dehydrogenase and Acetyl-CoA Synthase 111Holger Dobbek
Part 3: Assembly of Homometallic and Heterometalic Cu Cluster Active Sites 123
Assembly of Dinuclear Copper Center in Tyrosinases and Hemocyanins 125Nobutaka Fujieda and Shinobu Itoh
Multicopper Oxidases 139Daniel J. Kosman
Assembly of the Redox-Active Metal Centers of CytochromecOxidase 157Eva Nyvltova, Antoni Barrientos and Jonathan Hosler
CuA and CuZ Center Assembly in Nitrous Oxide Reductase 185Sofia R. Pauleta and Isabel Moura
MoCu CO Dehydrogenase and its Active-Site Assembly 197Frank Mickoleit
Part 4: Assembly of Homometallic and Heterometallic Mn Clusters 213
Homo- and Heterometallic Dinuclear Manganese Proteins: Active Site Assembly 215Gustav Berggren, Daniel Lundin and Britt-Marie Sjöberg
Biogenesis and Assembly of the CaMn4O5 Core of Photosynthetic Water Oxidases and Inorganic Mutants 233Colin Gates, Gennady Ananyev and G. Charles Dismukes
Part 5: Assembly of Homometallic and Heterometallic Ni Clusters 249
Urease Activation 251Robert P. Hausinger
Insights into [NiFe]-Hydrogenase Active Site Metallocluster Assembly 261Robert Gary Sawers and Constanze Pinske
Part 6: Assembly of Cofactors for Binding Active-Site Metal Centers 273
Moco in Mo/W Enzymes 275Silke Leimkühler
Heme Biosynthesis 299Amy E. Medlock and Harry A. Dailey
Siroheme Assembly and Insertion to Nitrite and Sulfite Reductase 315M. Elizabeth Stroupe and Martin J. Warren
Biosynthesis of Coenzyme F430 and the Posttranslational Modification of the Active Site Region of Methyl-Coenzyme M Reductase 323Steven O. Mansoorabadi, Kaiyuan Zheng and Phong D. Ngo
Coenzyme B12 Biosynthesis in Bacteria and Archaea 335Theodoric A. Mattes, Jorge C. Escalante-Semerena, Evelyne Deery and Martin J. Warren
Crosslinked CysTyr Free Radical Redox Cofactor 361James W. Whittaker
Topaquinone Biogenesis and Lysyl Tyrosine Quinone Biogenesis in Cu Amine Oxidases 375David M. Dooley, Doreen E. Brown and Eric M. Shepard
Index 389
