Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g., calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

InhaltsangabePart I. Introduction and Reviews CalciumBinding Proteins Hans J. Vogel, Richard D. Brokx, and Hui Ouyang Calcium Robert J. P. Williams Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, and John S. Elce The Multifunctional S100 Protein Family Claus W. Heizmann Ca2+ Binding to Proteins Containing g-Carboxyglutamic Acid Residues Egon Persson The Caseins of Milk as Calcium-Binding Proteins Harold M. Farrell, Jr., Thomas F. Kumosinski, Edyth L. Malin, and Eleanor M. Brown Part II. Calcium-Binding Proteins: Case Studies Preparation of Recombinant Plant Calmodulin Isoforms Raymond E. Zielinski Isolation of Recombinant Cardiac Troponin C John A. Putkey and Wen Liu Skeletal Muscle Troponin C: Expression and Purification of the Recombinant Intact Protein and Its Isolated N- and C-Domain Fragments Joyce R. Pearlstone and Lawrence B. Smillie Purification of Recombinant Calbindin D9k Eva Thulin S100 Proteins: From Purification to Functions Jean Christophe Deloulme, Gaëlh Ouengue Mbele, and Jacques Baudier Cadherins JeanRené Alattia, Kit I. Tong, Masatoshi Takeichi, and Mitsuhiko Ikura aLactalbumin and (CalciumBinding) Lysozyme Katsutoshi Nitta Recombinant Annexin II Tetramer HyoungMin Kang, Nolan R. Filipenko, Geetha Kassam, and David M. Waisman Purification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca2+-Binding Protein Mingjie Zhang and Kevin W.-H. Lo Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain V1 of Calpain Miroslaw Cygler, Pawel Grochulski, and Helen Blanchard Neurocalcin: Role in Neuronal Signaling Senadhi Vijay-Kumar and Vinod D.Kumar Crystallization and Structure-Function of Calsequestrin ChulHee Kang, William R. Trumble, and A. Keith Dunker Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains Eric A. Nalefski and Joseph J. Falke Ca2+Binding Mode of the C2ADomain of Synaptotagmin Josep Rizo, Josep Ubach, and Jesús García Study of Calcineurin Structure by Limited Proteolysis SeunAh Yang and Claude Klee

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